China Animal Husbandry & Veterinary Medicine ›› 2013, Vol. 40 ›› Issue (6): 60-64.

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Variance Analysis of Outer Membrane Protein of Three Reference Strains of Avibacterium paragallinarum Serotype A,B and C

YAO Dong-bi1,2,3, FANG Hui-ling1, SUN Hui-ling2,3   

  1. 1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;
    2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    3. Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Beijing 100097, China
  • Received:2012-11-29 Online:2013-06-20 Published:2013-06-20

Abstract: In order to explore the difference of outer membrane protein (OMP) of different serotype of Avibacterium paragallinarum, the OMP of three reference strains of Avibacterium paragallinarum serotype A (221),B(0222) and C (Modesto) were prepared by FOCUSTM Global Fractionation Kit and examined through SDS-PAGE. As a result, three different serotypes of Avibacterium paragallinarum showed similar SDS-PAGE profile. In each samples, there were seven main strips, each had the similar molecular weight which was analyzed by the gel quantitative software. The percentage of serotype A, B and C was 70.85%, 68.6% and 65.71%, respectively. In addition, the molecular weights of three bands were somewhat identical, whereas the protein content of them were significantly different (P<0.05). This study provided a theoretical basis for the further study of the OMP of Avibacterium paragallinarum.

Key words: Avibacterium paragallinarum; outer membrane protein; SDS-PAGE; quantitative gel

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