水貂肠炎细小病毒的分离鉴定及其VP2基因的序列分析

Abstract

Abstract: A virus was isolated from a dead mink which was suspected of being infected with the mink enteritis virus(MEV). It was eventually identified as MEV using PCR assay, cell culture and SDS-PAGE, and named MEV-WFD. The VP2 gene of this parvovirus isolate was cloned into pMD18-T vector, sequenced and analyzed. As a result,the VP2 protein possessed one sbustitution, 328A-T.The result of the nucleotide homologies of VP2 gene between this isolate and all MEV published on GenBank illustrated that MEV-WFD had the the highest homology 99.8% with ZYL-1,MEV/LN/-10 and Manzhouli.VP2 gene phylogenetic anaysis showed that this isolate and other six wild strains had the same ancestor.This research had laid the foundation for the mink enteritis parvovirus molecular epidemiological investigation.

Key words: mink enteritis virus; isolation and identification; sequence analysis

CLC Number:

S852.65⁺9.1

ZHANG Qing-ming, WANG Yu-ping, LI Ying-ying, YAN Xin-wu, LEI Lian-cheng. Isolation, Identification of Mink Enteritis Virus and Sequence Analysis of its VP2 Gene[J]. , 2013, 40(6): 26-30.

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Isolation, Identification of Mink Enteritis Virus and Sequence Analysis of its VP2 Gene

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