绒山羊皮肤干细胞的初步分离及碱性磷酸酶鉴定

Abstract

Abstract: The skin sample of cashmere goat was incubated in 0.02% trypsin at 4 ℃ overnight and the epidermis was separated,then they were placed in asterile tube containing 0.25% trypsin at 37 ℃ for 30 minutes and the dissociated cells were adhered with collagen type Ⅳ to culture dishes for 10 minutes. 10 minutes later, the unattached cells were removed and the rapidly adherent epidermal cells were cultured in stem cell growth medium(80% DMEM-F12 + 20% FBS + 25 μg/mL hydrocortisone + 100 IU/mL penicillin + 100 μg/mL streptomycin + 15 μg/mL insulin + transferring + 20 μg/mL EGF) for 24 h. And then dissociated cells were cultured on layer cells which the adult cashmere goat fibroblast were treated with 20 μg/mL mitomycin C for 4 h. After 2 weeks, these cells could form different shapes of clones. Dyed with AKP, it showed deep purple and cells were judged as positive.

Key words: cashmere goat; stem cell; isolation; identification

CLC Number:

S813.1⁺1

SU Xiao-hu, GAO Feng, LIU Ying-chun, WANG Hong, ZHANG Yan-ru, ZHOU Huan-min. Primary Isolation of Stem Cells from Cashmere Goat Skin and Identification of AKP[J]. , 2012, 39(10): 154-159.

References

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Primary Isolation of Stem Cells from Cashmere Goat Skin and Identification of AKP

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