China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (8): 154-156.

• 疾病防治 • Previous Articles     Next Articles

Determination of Content and Related Substances of Aspirin Eugenol Ester Microemulsion by RP-HPLC

WANG Qi-wen3，LI Jian-yong1，2， SHEN Xiao-yun3，YANG Ya-jun1，2，LI Bing1，2，WEI Xiao-juan1，2，ZHOU Xu-zheng1，2   

  1. (1.Lanzhou Institute of Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; 2.Key Laboratory of New Animal Drug Project, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; 3.Pratacultural Ecological Institute of Bijie University, Bijie 551700, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20
  • Contact: LI Jian-yong

Abstract: To determine the contents of Aspirin eugenol ester microemulsion(AEE-ME) and the related substances in AEE-ME, RP-HPLC method was used. The high performance liquid chromatographic conditions were as followed: ODS column (4.6 mm×100 mm,5 μm) was used as stationary phase and methanol-water (70∶30) was used as mobile phase; the detection wave length was 279 nm; lowing rate was 1.0 mL/min; temperature was 30 ℃. The calibration curves was a good linearity in the range of 1.25 to 40 μg/mL for AEE at 279 nm(r=0.9923),the average recovery rate was 99.93%, and RSD was0.84%. The results showed that the method was suitable for detection of the content and the related substances of Aspirin eugenol ester and could be used in quality control.

Key words: Aspirin eugenol ester; microemulsion; RP-HPLC; content determination

CLC Number: