Abstract: To obtain the recombinant vulpes vulpes growth hormone. The mature vulpes vulpes growth hormone mRNA obtained from pituitary was amplified by RTPCR , cloned into vector pMD18-T and subcloned into prokaryotic expression vector pPROEX^TMHta at restriction enzyme sites BamHⅠ and HindⅢ. The recombinant plasmids pMD18-TfGH cDNA and pPROEX^TMHta-fGH cDNA were identified by restriction enzyme digestion, PCR and sequencing. Then we transfer positive recombinant prokaryotic plasmid into E.Coil, and the protein was expressed under the induction of IPTG at the final concentration of 1 mmol/L. The protein expression was measured by SDS-PAGE, and a high level of fGH protein was obvserved at 26 kD which occupied about 35% of total E.coil protein. The recombined fGH protein existed in the for
m of inclusion bodies.

Key words: vulpes vulpes; growth hormone; cloning; prokaryotic expression