China Animal Husbandry & Veterinary Medicine ›› 2007, Vol. 34 ›› Issue (3): 50-52.

• 遗传繁育 • Previous Articles     Next Articles

Production Transgenic Pigs by Sperm-mediated Gene Transfer of Human her2 Gene

REN Hongyan, ZHANG Xingju, YANG Shulin, LI Xinyun, SHANG Tongling, JIN Erhui, TANG Zhonglin, CUI Wentao, LI Kui   

  1. Institute of Animal Science，Chinese Academy of Agricultural Science, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20

Abstract: The new collected semen removed the seminal fluid was mixed with the linearized plasmid DNA pro data, then stay it at 17℃for 2h let the DNA got into the sperm head. Sperm cells needed to penetrate and fertilize eggs by artificial insemination, the 2 pregnant gilts generated 20 piglets, and four positive transgenic pigs were detected by PCR. The research results proved that the human her2 gene had integrated into the pig chromosome, the integration rate was 20%. 

Key words: sperm-mediated gene transfer; transgenic pig; human her2 gene

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