猪瘟病毒E2蛋白A/D区单抗的制备及其抗原表位的初步研究

›› 2009, Vol. 36 ›› Issue (9): 50-54.

• 生物技术 • Previous Articles Next Articles

Preparation and Identification of Monoclonal Antibodies against the Truncated E2 Protein of Classical Swine Fever Virus

ZHANG Chun-ling¹, ZONG Xian-wei², YANG Wen-chao¹, ZHANG Jing-mei¹, PENG Ming-yi¹, CHENG Bao-yan³, YU Wei-yi¹

（1.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China; 2.Shandong Liuhe Poultry Company， Dezhou 253000, China; 3.Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
Contact: YU Wei-yi

Abstract

Abstract: A truncated gene encoding the major antigenic domains of E2 protein of classical swine fever virus (CSFV) was amplified and cloned into pGEX-4T-1 expression vector to obtain recombinant pGEX-4T-E（A）.The recombinant GST-E（A）protein expressed in E.coli Rosetta. BALB/c mice was immunized with purified GST-E（A）protein as antigen and mouse splenic cells were fused with SP2/0 cells. Hybridoma cells were screened by ELISA and subcloned. Two hybridoma cells C3 and A1 secreting anti-CSFV monoclonal antibodies were obtained. The ELISA showed the antibody titres were 1∶128000 and 1∶256000. Western blotting indicated that C3 and A1 reacted with CSFV specially. These results demonstrated that the McAbs were specific reagent for CSFV, and recognized a linear conserved epitope on the E2 protein.

Key words: classical swine fever virus; E2 protein; monoclonal antibody; identification

CLC Number:

S859.79⁺7

ZHANG Chun-ling;ZONG Xian-wei;YANG Wen-chao;ZHANG Jing-mei;PENG Ming-yi;CHENG Bao-yan;YU Wei-yi. Preparation and Identification of Monoclonal Antibodies against the Truncated E2 Protein of Classical Swine Fever Virus[J]. , 2009, 36(9): 50-54.

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Preparation and Identification of Monoclonal Antibodies against the Truncated E2 Protein of Classical Swine Fever Virus

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