China Animal Husbandry & Veterinary Medicine ›› 2008, Vol. 1 ›› Issue (5): 25-27.

• 生物技术 • Previous Articles     Next Articles

Clone and Sequence Analysis of Variable Region Gene of a McAb Against NDV HN Protein

LU Xishan1,HUANG Yanyan2,HU Beixia2,LI Shijuan4,YU Zhou1,LI Xiangzhao1,WANG Jinbao3,ZHANG Xiumei2   

  1. 1.College of Animal Science and Veterinary Medicine, Qingdao Agriculture University, Qingdao 266109, China;2.Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan 250000, China;3.Shandong Academy of Agricultural Science, Jinan 250000, China;4. Agriculture Development Office of Jinan City, Jinan 250000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-05-20 Published:2008-05-20

Abstract: With the total RNA from C3-B7 hybridoma cells which secrete monoclonal antibody against NDV HN protein, the VH(heavy-chain variable region) and VL(lightchain variable region)genes encoding the monoclonal antibody were amplified by RT-PCR. The purified pruduct was cloned to pMD18-T vector and the recombined plasmids were transformed into DH5α. Colonies were selected and the specific recombinant plasmid was identified by colony PCR. The result shows that the VH gene encoding 119 aa and the VL gene encoding 110 aa were 357 bp and 332 bp, respectively.This establish the foundation for the construction and expression of single chain Fv(svFv).

Key words: newcastle disease virus; HN protein; variable region gene; clone; sequence analyse

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