Abstract: In this study, the total RNA was isolated from Guangxi Bama mini-pig
ovary tissue with RNAwatson extraction. The complementary DNA (cDNA) encoding follistatin protein was ampilified by the reverse transcription polymerase chain reaction (RT-PCR) method and a pair of differential primers. The amplified cDNA fragment was inserted into pMD18-T vector. The recombined vectors were verified through the sequence survey. The results showed that the cloned follistatin cDNA was highly homologous with the reported nucleotide encoding swine follistatin in GenBank(99.4%). Meanwhile, the identified follistatin cDNA was inserted in the multiple cloning sites of plasmid pET 32a+ to construct prokaryotic expression vector, the latter was transformed into the E coli BL21 induced by IPTG. Then a fusion protein was obtained (about 58.4 ku) in SDSPAGE and Western blotting.These results showed that the follistatin cDNA was expressed in prokaryotic cells.

Key words: Guangxi Bama mini-pig; follistatin; cloning; prokaryotic expression