China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (6): 91-94.

• 生物技术 • Previous Articles     Next Articles

Preparation of Rabbit Anti-goose IgG-HRP and Preliminary Application

LIU Fei1,2, TANG Wei-jie1, HAN Qing-lin1,3, ZHAO Ting-ting1, GAO Li-qin1, YAN Xiao-ling1   

  1. 1. College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China;2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014,China;3. Bureau of Animal Husbandry of Daying, Sichuan Province, Daying 629300, China
  • Received:1900-01-01 Revised:2011-03-27 Online:2011-06-20 Published:2011-06-20

Abstract: To study on preparation of rabbit anti-goose IgG-HRP, the experimentation isolated IgG from the goose egg yolk, and the method was employed to dilute with water-n-octylic acid-ammonia sulfate initial extraction, out salt dialysis and DEAEA-50 chromatography. Protein nucleic acid detector and SDS-PAGE indicated the IgG concentration; the purified IgG was labeled with HRP; effect of GPV VP3 gene vaccine was detected with the labeled antibody. The results indicated that the initial extraction was 10 mg/mL and the purity was 94.5%; the antiserum characterized by immunodiffusion showed that the titer of the antiserum was 1∶32 and specificity of the antiserum was high. The titer of labeled antibody was 1∶3000; the labeled antibody was a good tool to detect the effect of GPV VP3 gene vaccine.

Key words: goose egg yolk IgG; isolation; enzyme labeling; pcDNA-VP3 DNA vaccine

CLC Number: