China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (9): 237-240.

• 经验交流 • Previous Articles    

Development and Application of Dot-ELISA for Rapid Detection of Gentamicin Residue in Milk

CHEN Yan1, LIANG Chi-zhou1,2, WU Bin3, ZOU Ming-qiang1, XUE Qiang1, GUO Liang-hong2

  

  1. (1.Chinese Academy of Inspection and Quarantine, Beijing 100123, China; 2.Research Center for Eco-Environmentd Scienses, Chinese Academy of Sciences, Beijing 100085, China; 3. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
  • Contact: ZOU Ming-qiang

Abstract: A dot enzyme linked immunosorbent assay (Dot-ELISA) was established for rapid detection of gentamicin residue in milk on-site. The Dot-ELISA was performed on modified nitrocellulose membrane strip (NCM strip) which had eight round pieces of nitrocellulose membrane with diameter of 2 mm, which was cut by Versa Laser, and then was affixed on a plastic adhesive tape. Based on the NCM strip, a direct competitive Dot-ELISA was developed for detecting gentamicin and the influence of several factors on the immunoassay was studied. The optimized Dot-ELISA has been used to detect gentamicin in milk and a limit detection of 60 ng/mL was obtained. Compared with the normal ELISA tests carried on the microtiter plates, the detection time was saved evidently. The Dot-ELISA was characterized by easy use, rapid reaction, economical feasibility, and observable results and the method was worth promoted.

Key words: gentamicin; Dot-ELISA; NCM; rapid detection

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