China Animal Husbandry and Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (5): 2078-2087.doi: 10.16431/j.cnki.1671-7236.2025.05.013

• Nutritionand Feed • Previous Articles    

Establishment and Evaluation of Double Cannulas Model of Duodenal and Terminal Ileum of Bama Minipigs

QIN Xiyu1, LIU Xiaoxue2, CHAI Yi3, LAI Mengxuan4, REN Xiaomin4, ZHANG Depeng5, ZHANG Peng5, LIJuntao6, LI Yixuan2, WANG Ran2, HAO Yanling2, WU Huijuan5, WANG Xiaoyu1   

  1. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
    2. Department of Nutrition and Health, China Agricultural University, Beijing 100193, China;
    3. College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China;
    4. Mengniu Hi-Tech Dairy Products (Beijing) Co., Ltd., Beijing 101100, China;
    5. Beijing Laboratory Animal Research Center Co., Ltd., Beijing 102600, China;
    6. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:2024-08-15 Published:2025-04-27

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Abstract: 【Objective】 To address the issues of excessive body weight,easy detachment of cannulas and the inability to separately investigate the roles of gastric and intestinal parts in digestion and absorption in the existing single-cannulas animal models,the double cannulas model of duodenum and terminal ileum in Bama Minipigs was established to investigate the feasibility and effectiveness of double-cannulas models of the animal intestine in the experiments related to the gastrointestinal digestion and absorption of nutrients and medicines.【Method】 A total of 11 nine-month-old Bama Minipigs (17.5 kg±2.0 kg) were selected for this study.After anesthesia,an incision was made in the right abdominal region to expose the intestines,followed by purse-string suturing.The cannulas were inserted and fixed,and the incisions were closed.Postoperative care was carefully managed to prevent infection.Once the animals had fully recovered,the experiment was conducted using direct ultra-high temperature (dUHT) milk as a test substance.Intestinal effluents and blood samples were analyzed using SDS-PAGE,confocal laser scanning microscopy (CLSM),and ultra-performance liquid chromatography (UPLC) to detect changes in protein and amino acid content.The dynamic changes in milk protein during digestion and absorption were assessed to confirm the reliability and applicability of the model for simulating food digestion and absorption.【Result】 All 11 experimental animals survived for two months post-surgery without any significant discomfort or complications,and showed good growth,with an average weight gain of 12.23 kg,consistent with normal growth trends.During the experimental period,no cannulas detachment was observed,and the cannulas functioned normally,enabling the smooth collection of duodenal and ileal effluents as well as blood samples.In this experiment,the protein microstructure of the effluent samples was examined,and it was observed that the protein content gradually decreased with the digestion time continuously increased.During digestion,casein and β-lactoglobulin bands showed a tendency of enhancing and then weakening.Detecting the amino acid content in the blood at different time points before and after ingestion,and the amino acid absorption curves were obtained.The incremental area under the curve (iAUC) of total amino acid in plasma was (12 105.00±2 145.57) μg·min/mL after ingestion,which was consistent with the normal kinetics of milk protein absorption.【Conclusion】 This study successfully established the double cannulas model involving the duodenum and the terminal ileum in Bama Minipigs,validating the effectiveness of the surgical technique and postoperative care regimen.The double cannulas model demonstrated good application prospects in Bama Minipigs,providing a reliable experimental animal model for studies on gastrointestinal digestion and absorption.This model offered strong support for exploring the digestion and absorption of nutrients,new drug development,and evaluating the absorption of existing drugs.

Key words: Bama Minipigs; double intestinal cannulas; digestive absorption; postoperative care

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