不同浓度鞣花酸对猪精液常温保存效果的影响

doi:10.16431/j.cnki.1671-7236.2024.10.019

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effects of ellagic acid (EA) on the quality and antioxidant properties of boar semen during room temperature preservation，in order to provide a theoretical basis for the promotion and application of EA in semen preservation.【Method】 Semen was collected from four healthy adult Duroc boars aged 2 to 3 years. The qualified semen was divided into five portions and diluted with BTS (Beltsville thawing solution) diluted at room temperature，which contained different concentrations of EA (0 (control),5，10，15 and 20 μg/mL). Sperm motility，average path velocity，swing amplitude，whipping frequency，plasma membrane integrity rate，acrosome integrity rate，and antioxidant capacity were assessed on the 1st，3rd，5th，and 7th days of preservation. 【Result】 On the 1st day of preservation，plasma membrane integrity was significantly higher in 15 μg/mL EA group compared to control group (P＜0.05)，and there was no significant difference in other indicators among experimental groups and control group (P＞0.05). On the 3rd day of semen preservation，the average path velocity，catalase (CAT) activity，and total antioxidant capacity (T-AOC) of sperm in 10 and 15 μg/mL EA groups were significantly higher than those in control group (P＜0.05). On the 5th day of semen preservation，the swing amplitude of sperm in 15 and 20 μg/mL EA groups was significantly lower than that in control group and the 5 and 10 μg/mL EA groups (P＜0.05)，and the sperm motility in 5，10 and 15 μg/mL EA groups was significantly higher than that in control group (P＜0.05). The CAT，glutathione peroxidase (GSH-Px) activitives，and T-AOC in the semen of 10，15 and 20 μg/mL EA groups were significantly higher than those in control group (P＜0.05). On the 7th day of semen preservation，the sperm motility，average path velocity，plasma membrane integrity，acrosome integrity，CAT activity，and T-AOC in each experimental group were significantly higher than those in control group (P＜0.05). 【Conclusion】 The incorporation of EA into BTS dilution solution was found to offer a protective effect on semen during room-temperature storage，with the optimal outcome observed at an EA concentration of 10 μg/mL.

Key words: ellagic acid; room temperature preservation; semen quality; antioxidant ability; pig

CLC Number:

S814.3

QU Xianxian, SHU Liang, SUN Xiaojing, LIU Mei, GAO Fengxian, YANG Tai. Effect of Different Concentrations of Ellagic Acid on Preservation of Pig Semen at Room Temperature[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4383-4390.

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Effect of Different Concentrations of Ellagic Acid on Preservation of Pig Semen at Room Temperature

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