Abstract: In order to highly express the ideal Rhipicephalus microplus antigen, we analyzed genetic evolution of Bm86 gene on the laboratory acquired in this study and biological information of its coding protein. The results showed that the homologies of complete open reading frame sequences of nucleotide and amino acid sequence of Bm86 gene of Rhipicephalus microplus with all the foreign Bm86 gene logged in GenBank were 95.9% to 98.6% and 93.8% to 97.8%，respectively. The difference was very small. The evolutionary tree showed that the strain and most of logged strains were in same branch. Biology information prediction showed that the protein was unstable. Insoluble probability was 93.26%. There were 4 potential glycosylation sites. Analysis of the above information would lay the foundation for the gene expression, preparation of immune antigen and development of vaccine against Rhipicephalus microplus.

Key words: Rhipicephalus microplus; Bm86 gene; Bm86 protein