Abstract: The aim of this research was to study the mechanisms of Lactobacillus amylovorus metabolites on bacteriostasis and antiviral activity，and identify the active ingredients of bacterial metabolites.The supernatants were treated by acids，hydrogen peroxide and protease.The agar well diffusion system was performed for detecting the bacteriostasis activity of the supernatants of Lactobacillus amylovorus against Escherichia coli，Salmonella and Staphylococcus aureus.Then evaluated the anti-TGEV activity on the IPEC-J2 cells with MTT.The results showed that the bacteriostasis of the supernatants of Lactobacillus amylovorus reduced significantly by treating with hydrogen peroxide，trypsin and pepsin （P<0.05），but antiviral activity was not reduced significantly （P>0.05）.However，the bacteriostasis and antiviral activity of the supernatants of Lactobacillus amylovorus reduced significantly by treating with acids and protease K （P<0.05），it was found that the bacteriostasis and antiviral activity of Lactobacillus amylovorus metabolites possess consistency and the component was acids or protease K-sensitive proteins.

Key words: Lactobacillus amylovorus; antivirus; TGEV; IPEC-J2