马兜铃酸肾病中相关因子的表达变化

Expression Changes of Relative Factors in Mice with Aristolochic Acid Nephropathy

TIAN Ya-hui¹, CHANG Di¹, YANG Yao-hui¹, WU Guo-ying², ZHAO Hai-jiao¹, WU Jin-dong¹, WU Guo-juan¹, ZHANG Zhong-wen¹

1. Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China; 2. Maternity and Child Care Center, Shuangyang District, Changchun City, Jilin Province, Changchun 130600, China

Received:2014-04-29 Online:2014-08-20 Published:2014-08-22

Abstract

Abstract: The study was aimed to observe the expression changes of relative factors in mice with aristolochic acid nephropathy (AAN). 30 mice were given aristolochic acid Ⅰ (AAⅠ) at 8 mg/(kg·d), and mice were killed at a different time. Then observe the renal pathological changes by hematoxylin-eosin (H&E), Real-time PCR and Western blotting technology were used to detect the expression changes of α-SMA, vimentin and CK-18. The results showed that with the extension of treatment time, we could found the kidney structure was damaged more seriously, and confirmed that AAⅠcould induce epithelial-mesenchymal transition (EMT) in renal, promoted the expression of α-SMA and vimentin, inhibited the expression of CK-18. The study laid a foundation for a deeper mechanism study of AAN.

Key words:

aristolochic acid nephropathy; α-SMA; vimentin; CK-18

TIAN Ya-hui, CHANG Di, YANG Yao-hui, WU Guo-ying, ZHAO Hai-jiao, WU Jin-dong, WU Guo-juan, ZHANG Zhong-wen. Expression Changes of Relative Factors in Mice with Aristolochic Acid Nephropathy[J]. .

References

1 任智星，马宏，崔群玮，等. 波形蛋白在肾间质纤维化大鼠肾组织中表达趋势及螺内酯干预的研究［J］. 中国药物与临床，2012，12(5)：588～590.
2 阳晓，曾又佳. 应重视马兜铃酸肾病易感机制的研究［J］. 中国中西医结合肾病，2010，11(3)：189～191.
3 陆红，胡丽萍，洪丹，等. 马兜铃酸诱导大鼠肾小管上皮细胞表型转化和胶原累积及垂盆草提取物的作用［J］. 中国病理生理杂志，2013，29(12)：2172～2178.
4 赵海焦，高磊，陆彦，等. Smad泛素化调节因子2 siRNA的筛选及转染原代肾小管上皮细胞条件的优化［J］. 中国畜牧兽医，2014，41(1)：19～24.
5 彭晓兰，董晓凯，廖芳芳，等. 马兜铃酸诱导肾小管上皮细胞凋亡的检测［J］. 中国畜牧兽医，2011，38(11)：76～80.
6 韩敏，徐钢，曾锐，等. B-连环素酪氨酸磷酸化调控E-钙粘蛋白表达参与转化生长因子-B1诱导的人肾小管上皮细胞转分化［J］. 中华肾脏病杂志，2006，22(8)：494～498.
7 Boyer B, Valles A M, Edme N. Induction and regulation of epithelial-mesenchymal transitions［J］. Biochem Pharmacol, 2000, 60: 1091～1099.
8 Debelle F D, Vanherweghem J L, Nortier J L. Aristolochic acid nephropathy: A worldwide problem［J］. Kidney Int, 2008, 74(2): 158～169.
9 Gokmen M R, Lord G M. Aristolochic acid nephropathy［J］. British Medical Journal, 2012, 344： e4000.
10 〖ZK(#〗Li J, Zhang Z, Wang D, et al. TGF-beta 1/Smads signaling stimulates renal interstitial fibrosis in experimental AAN［J］. Journal of Receptors and Signal Transduction, 2009, 29(5): 280～285.
11 Li W, Jiang H, Feng J M. Isogenic mesenchymal stem cells transplantation improves a rat model of chronic aristolochic acid nephropathy via upregulation of hepatic growth factor and down regulation of transforming growth factor 1［J］. Mol Cell Biochem, 2012, 368(1～2): 137～145.
12 Nortier J L, Martinez M C, Schmeiser H H, et al. Urothelial carcinoma associated with the use of a Chinese herb［J］. N Engl J Med, 2000, 342: 1686～1692.
13 Thiery J P. Epithelial-mesenchymal transition in development and pathologies［J］. Curr Opin Cell Biol, 2003, 15: 740～746.
14 Vanherweghem J L, Depierreux M, Tielemans C, et al. Rapidly progressive interstitial renal fibro sis in young women: Association with slimming regimen including Chinese herbs［J］. Lancet, 1993, 341(8842): 387～391.
15 Wang Y, Zhang Z, Shen H, et al. TGF-β1/Smad7 signaling stimulates renal tubulointerstitial fibrosis induced by AAI［J］. Journal of Receptors and Signal Transduction, 2008, 28(4): 413～428.
16 Zhou L, Fu P, Huang X R, et al. Mechanism of chronic aristolochic acid nephropathy: Role of Smad3［J］. American Journal of Physiology-Renal Physiology, 2010, 298(4): F1006～F1017.

Expression Changes of Relative Factors in Mice with Aristolochic Acid Nephropathy

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