牛瘤胃乳酸菌的分离与鉴定

Abstract

Abstract: To screen and separate viable preparations for bovine strains of lactic acid bacteria，this separated lactic acid bacteria from bovine rumen were identified by API 50CHL.For biochemical identification of strains by resistance for oxygen，acid and alkali，determination of the growth temperature, drug resistance and antibacterial property.The results showed that the three strains of lactic acid bacteria have been identified，which were L. acidophilus （L1）, L. fermentum （L2） and L. holzapfelii （L3）, respectively. L1 had strongest acid，L2 had strongest alkali，L1 was strong in 37 to 40 ℃ were grown between good and facultative anaerobic，the drug resistance rate of L1，L2 and L3 was 60% to 100%.The three strains compare with the Escherichia coli and Staphylococcus showed their inhibition，the L1 had the best bacteriostatic effect，the antibacterial diameter of 18.30 and 16.40 mm，respectively.In conclusion，L1 and L2 will be a candidate viable lactic acid bacteria strains resistant formulations.

Key words:

lactic acid bacteria；antibacterial property；biochemical characteristics；rumen

YANG Long-long，JIANG Jian-jun，YAN Gen-qiang，WANG Jing-mei，GAO Jia-deng，WANG Chao-li，YANG Ming-wei. Isolation and Identification of Lactic Acid from Bovine Rumen[J]. .

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Isolation and Identification of Lactic Acid from Bovine Rumen

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