添加不同乳酸菌剂对苜蓿青贮营养价值的影响

Abstract

Abstract: This experiment was conducted to investigate the influence of adding different lactic acid bacteria inoculants on nutrient values of alfalfa silage.The test took the alfalfa as material while adding Lactobacillus casei（A-LAB），Lactobacillus plantarum（B-LAB） and Pediococcus pentosaceus （C-LAB） at dose of 10⁶ CFU/g fresh weight to ensile and set the control group，respectively.The cornell net carbohydrate and protein systems （CNCPS） was used to evaluate nutrient values of alfalfa silage after ensiling 45 days.The results showed that adding lactic acid bacteria inoculants could decrease the content of non-protein nitrogen （PA） compared with the control group. and the effect of B-LAB reached to the significant level （P＜0.05）.The content of intermediately degraded protein （PB₂） and slowly degraded protein （PB₃） of all the lactic acid bacteria inoculant groups were higher than the control group，but the difference was not significant （P＞0.05）.In the aspects of carbohydrate fractions，adding lactic acid bacteria inoculant increased the content of sugars （CA） and non-structural carbohydrate （CNSC） and decreased the content of available fiber （CB₂），and the effect of A-LAB reached to the significant level （P＜0.05）.In conclusion，adding lactic acid bacteria inoculant could improve the nutrient values of alfalfa silage to certain extent by preserving the true protein and increasing the content of rapid degradation rates and CNSC of carbohydrate fractions，and Lactobacillus plantarum and Lactobacillus casei had the better effect.

Key words: alfalfa silage; lactic acid bacteria; CNCPS; nutrient value

LAI Yu-jiao，LUO Hai-ling，WANG Zhen-zhen，LU Xiao-nan，QU Yang-hua，GAO Wei-min，LIU Kun，YU Zhu. Effect of Adding Different Lactic Acid Bacteria Inoculants on Nutrient Values of Alfalfa Silage[J]. .

References

1 万志平，郭继晓，苏金文，等.紫花苜蓿青贮制作试验［J］.中国奶牛，2011（15）：50～52.
2 王莹，玉柱.不同添加剂对紫花苜蓿青贮发酵品质的影响［J］.中国草地学报，2010，32（5）：80～84.
3 王海林，陈凤林，马慧.乳酸菌制剂对去穗玉米秸青贮营养成分及其奶牛瘤胃降解率的影响［J］.中国乳业，2013（5）：26～29.
4 王燕，杨方，陈常栋，等.康奈尔净碳水化合物—蛋白质体系预测小肠可消化粗蛋白质含量［J］.动物营养学报，2012，12（7）：1274～1282.
5 曲永利，吴健豪，李铁.应用康奈尔净碳水化合物—蛋白质体系评定东北农区奶牛饲料营养价值［J］.动物营养学报，2010，22（1）：201～206.
6 李改英，高腾云，傅彤，等.影响苜蓿青贮的因素及其青贮技术的研究进展［J］.中国畜牧兽医，2010，37（12）：22～26.
7 李建云，柴贵宾，朱晓萍，等.辽宁绒山羊常用饲料营养价值评定［J］.中国畜牧杂志，2012，48（7）：38～42.
8 张宁，张永根，李欣新，等.添加2种乳酸菌制剂对水稻秸青贮品质及有氧稳定性的影响［J］.中国畜牧杂志，2013，49（5）：72～75.
9 张金霞，乔红霞，刘雨田.水分和添加剂对紫花苜蓿青贮品质的影响［J］.草业科学，2014，31（4）：766～770.
10 陈韵虹，赵钢，余土元.不同青贮处理对3种青贮饲料CNCPS蛋白质组分的影响［J］.仲恺农业工程学院学报，2013，26（3）：13～17.
11 周俊华，邹彩霞，梁贤威，等.应用康奈尔净碳水化合物—蛋白质体系评定广西水牛常用粗饲料的营养价值［J］.动物营养学报，2011，23（12）：2190～2197.
12 赵广永.反刍动物营养［M］.北京：中国农业大学出版社，2012.
13 席兴军，韩鲁佳.添加乳酸菌和纤维素酶对玉米秸秆青贮饲料品质的影响［J］.中国农业大学学报，2003，8（2）：21～24.
14 徐炜，师尚礼，祁娟，等.不同添加剂对夏河县紫花苜蓿青贮品质的影响［J］.草地学报，2014，22（2）：414～419.
15 贾娅敏，银欢，高强，等.乳杆菌对紫花苜蓿青贮品质的影响［J］.西南农业学报，2013，26（6）：2601～2604.
16 程方方，杨连玉，许雪魁，等.糖蜜和乳酸菌对光叶紫花苕青贮品质的影响［J］.草地学报，2012，20（5）：947～951.
17 Albrecht K A，Muck R E.Proteolysis in ensiled forage legumes that vary in tannin concentration［J］.Crop Science，1991，31（2）：464～469.
18 Berthiaume R，Benchaar C，Chaves A V，et al.Effects of nonstructural carbohydrate concentration in alfalfa on fermentation and microbial protein synthesis in continuous culture［J］.Journal of Dairy Science，2010，93（2）：693～700.
19 Brito A F，Tremblay G F，Bertrand A，et al.High total nonstructural carbohydrates timothy enhanced performance of midlactation dairy cows［J］.J Anita Sci，2010，24：399.
20 Contreras-Govea F E，Muck R E，Broderick G A，et al.Lactobacillus plantarum effects on silage fermentation and in vitro microbial yield［J］.Animal Feed Science and Technology，2013，179（1）：61～68.
21 Guo X S，Ding W R，Han J G，et al.Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives［J］.Animal Feed Science and Technology，2008，142（1）：89～98.
22 Krishnamoorthy U，Sniffen C J，Stern M D，et al.Evaluation of a mathematical model of rumen digestion and an in vitro simulation of rumen proteolysis to estimate the rumen-undegraded nitrogen content of feedstuffs［J］.British Journal of Nutrition，1983，50（3）：555～568.
23 Lou〖AKcˇ〗ka R.Fractions of protein and fibre of alfalfa［A］.14th International Symposium Forage Conservation［C］.2010.
24 McKersie B D.Effect of pH on proteolysis in ensiled legume forage［J］.Agronomy Journal，1985，77（1）：81～86.
25 Pichard G，Van Soest P J.Protein solubility of ruminant feeds［M］.Ithaca NY：Proc Cornell Nutr Conf，1977.
26 Rooke J A，Armstrong D G.The importance of the form of nitrogen on microbial protein synthesis in the rumen of cattle receiving grass silage and continuous intrarumen infusions of sucrose［J］.British Journal of Nutrition，1989，61（1）：113～121.
27 Smith L W，Goering H K，Gordon C H.Relationships of forage compositions with rates of cell wall digestion and indigestibility of cell walls［J］.Journal of Dairy Science，1972，55（8）：1140～1147.
28 Sniffen C J，O’connor J D，Van Soest P J，et al.A net carbohydrate and protein system for evaluating cattle diets：Ⅱ.Carbohydrate and protein availability［J］.Journal of Animal Science，1992，70（11）：3562～3577.
29 Van Soest P J，Sniffen C J，Mertens D R，et al.A net protein system for cattle：The rumen submodel for nitrogen［A］.Protein Requiremnts for Cattle：Proceedings of an International Symposium［C］.1981.

[1]	CHENG Zeng, ZHANG Xiaojuan, TANG Fu, HE Ganggang, GAO Xinmei, GAO Wei. Prediction of Ruminal Degradability of Total Mixed Ration Carbohydrate in Sheep by CNCPS-S Model [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2423-2432.
[2]	XU Hongjian, ZHANG Liyang, LIU Xin, JIANG Xin, LI Xin, ZHANG Yonggen. Study on the Correlation Between the CNCPS Fractions and Furosine Content in DDGS with Different Heat Treatment [J]. , 2018, 45(12): 3471-3478.
[3]	LI Yan, MENG Qing-xiang, CHEN Wan-bao, LIU Chang, ZHOU Zhen-ming. Evaluation of the Nutritional Value of Soybean Curb Residues by Cornell Net Carbohydrate and Protein System and Gas Production Technique [J]. , 2017, 44(5): 1355-1362.
[4]	LI Yan, YANG Jun-xiang, MENG Qing-xiang, LIU Chang, CHEN Wan-bao, ZHOU Zhen-ming. Evaluation the Potential of Apple Pomace as Ruminant Non-grain Feed by Cornell Net Carbohydrate and Protein System and Gas Production Technique [J]. , 2017, 44(4): 1001-1008.
[5]	HUANG Shuai, ZHU Fei, WANG Shang, ZHAO Nan, CHENG Jian-bo, ZHANG Yang-dong, WANG Hong-rong, FAN Cai-yun. Evaluation of Nutritive Value of Unconventional Feed in Anhui Province and the Surrounding Areas Using CNCPS [J]. , 2016, 43(5): 1385-1391.
[6]	ZHAO Yun-jun;LI Xue-sen;DONG Zhi-guo;AN Deng-di. Selection of Lactic Acid Bacteria(LAB) from Previously Fermented Juice and Alfalfa Silage and its Character [J]. , 2011, 38(1): 29-32.
[7]	XIE Xiang-xue;MENG Qing-xiang;DAI Jun-fang;REN Li-ping;. Research Progress and Development Trend of Cornell Net Carbohydrate and Protein System in China [J]. , 2010, 37(5): 17-20.
[8]	LI Gai-ying;GAO Teng-yun;FU Tong;LIAN Hong-xia;CHEN Yu-xia. Factors Affecting Alfalfa Silage and Research Progress on Alfalfa Silage Technology [J]. , 2010, 37(12): 22-26.

Effect of Adding Different Lactic Acid Bacteria Inoculants on Nutrient Values of Alfalfa Silage

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments