结核分枝杆菌rv0199基因在耻垢分枝杆菌中的表达及检测

Abstract

Abstract: To study the role of Mycobacterium tuberculosis(M.tb) rv0199 gene may play in the pathogenicity of the pathogen, rv0199 gene was cloned, the protein was heterologous expressed in M.smegmatis(Ms) and its expression was detected. The E.coli-Mycobacteria shuttle vector pMV261 was improved by introducing the commonly used 6His and Strep Ⅱ recombinant protein tags, the improved vector was named as pMV262. The rv0199 gene was over-expressed in Ms/pMV262 expression system, the recombinant protein was specifically detected by the anti-6His tag antibody and anti-Strep Ⅱ tag antibody. The heterologous expression of M.tb gene in Ms could be easily detected by the shuttle vector pMV262 constructed in this study, without preparing monoclonal or polyclonal antibody against the protein. The recombinant bacteria Ms/262-99 provided material and laid foundation for further study of the potential function of rv0199 gene.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Mycobacterium tuberculosisfont-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">; rv0199 gene; pMV261; pMV262; M.smegmatis

CUI Bao-liang, CHEN Li-ping, ZHANG Luan-luan, LI Hua-fang, LIU Si-guo. Expression and Detection of Mycobacterium tuberculosis rv0199 Gene in Mycobacterium smegmatis[J]. .

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Expression and Detection of Mycobacterium tuberculosis rv0199 Gene in Mycobacterium smegmatis

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