绵羊肺炎支原体热休克蛋白Hsp70的生物信息学分析

Abstract

Abstract: The assay was aimed to establish the ELISA by Mycoplasma ovipneumoniae(Mo) strain Y98 Hsp70 protein which biological characteristics should be analyzed.The physical and chemical parameters,transmembrane,signal peptide,secondary structure,B cell epitopes were predicted and analyzed by DNAStar,Bioedit 7.0,ClustalX 3.0,Mega 4.0 softwares and Protparam,TMpred,IEDB online tools in this study. The results showed that the Hsp70 protein of Mo strain Y98 was stable and soluble which predicted molecular weight was 66.14 ku and pI was 5.08. The protein had no transmembrane and did not secrete signal peptide. It was also proved that the protein was ideal target protein to establish immune method due to the advantage structure of epitope existed. It was indicted that the evolutionary relationships between Mo and Mycoplasma hyopneumoniae was close which was far compared with Mycoplasma mycoides cluster by constructed phylogenetic trees of Hsp70 gene. It would provide theoretical basis to analyze pathogenesis of Mo further.

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ZHANG Shuang-xiang，CHENG Zhen-tao，TANG Yu，RAN Long-zhong，LI Tao，ZHOU Bi-jun，WEN Ming，WANG Kai-gong，WANG Hui. Bioinformatics Analysis of Hsp70 Gene of Mycoplasma ovipneumoniae[J]. .

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Bioinformatics Analysis of Hsp70 Gene of Mycoplasma ovipneumoniae

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