1株Ⅶ型新城疫病毒融合蛋白基因的克隆与生物信息学分析

Abstract

Abstract: The assay was aimed to analyze the protein structure and function of fusion(F) gene of genotype Ⅶ Newcastle disease virus(NDV) strain CY1. The total RNA was extracted from NDV CY1 strain, F gene was amplified by RT-PCR and sequenced, then using some related computer software and database, the sequence of F gene of NDV CY1 strain and the deduced amino acid sequence were analyzed, and the secondary structure and hydrophobicity of the protein were determined and predicted. The results showed that F gene of NDV CY1 strain was consisted of 1662 nucleotides encoding a 553-amino acid protein. The protein had two transmembrane domains, and its subcellular localization was in plasma membrane and endoplasmic reticulum. The secondary structure contained several kinds of conformations. Grand average of hydropathicity of F protein of NDV CY1 strain was 0.174. The theoretical pI, estimated half-life, instability index and fatty index of F protein was 8.48, 30 h （mammalian reticulocytes in vitro）,31.62 and 108.12, respectively. The maximum and minimum hydropathicities were 3.078 and -2.578，respectively. The protein included a 31-amino acid signal peptide.Motif search showed that F protein had 6 N-glycosylation site, 9 casein kinase Ⅱ phosphorylation site, 7 protein kinase C phosphorylation site, 14 N-myristoylation site,1 amidation site,1 leucine zipper pattern,1 microbodies C-terminal targeting signal, 1 bipartite nuclear localization signal profile. Analysis of phylogenetic tree indicated that the F gene of NDV CY1 strain had the highest genetic relationship with zj1 strain.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Newcastle disease virus; F gene; bioinformatics analysis

WU Yun-pu ZHAO Xiao-tong, XUE Shu-shan SHI Lin, HE Li-kun, ZHANG Xiao-hua, CAO Ming-hui, GU Gui-bo, GU Zhi-da, WEI Shu, CAO Dong. Cloning and Bioinformatics Analysis of F Gene of Genotype Ⅶ Newcastle Disease Virus[J]. .

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Cloning and Bioinformatics Analysis of F Gene of Genotype Ⅶ Newcastle Disease Virus

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