Abstract: Analysing the new gene of E. acervulina sporulated oocysts stage by clone and bioinformatics technology. We acquired the full-length cDNA of EST sequence by RACE technique based on the cDNA expression library of E. acervulina. The cDNA of EST sequence coded protein was analysised and predicted by several bioinformatics software, the result showed the EST sequence was a new gene of E. acervulina sporulated oocysts stage, the GenBank ID was EU590120. The EST sequence including a 492 bp open reading frame which coded 163 amino acids residues,the theory of molecular weight was 17.049 ku, isoelectric point was 6.69, 8 acidic amino acids(Asp + Glu), 8 basic amino acids(Arg and Lys)，molecular formula was C₇₆₁H₁₂₂₃N₁₉₉O₂₁₉S₁₂, the total average hydrophilic(GRAVY) was 0.731. The signal peptide analysis showed that the ORF encoding protein was an secretory protein containing a signal peptide and four transmembrane structures, its the amino acids residues between 105 to 115, 28 to 32 and 132 to 138 had strong hydrophobicity. At the same time, the protein contains one protein kinase C phosphorylation sites, one N-glycosylation sites, two casein kinase Ⅱ phosphorylation site, four N-end nutmeg acetoxylation site. The bioinformatics analysis showed that the ORF encoding protein possess several biological function sites and potential antigen epitopes.

Key words:

chicken；coccidia；Eimeria acervulinamso-bidi-font-family: 宋体">；new gene；cloning；bioinformatics