Abstract: The assay was aimed to obtain the best extraction conditions of the yellow oral liquid. The article determinated the content of berberine hydrochloride in the yellow oral liquid in different extraction conditions by UV method, selected the highest content extraction conditions as the best ones. A good linear correlation of berberine hydrochloride was observed within the range of 1.5625 to 25 μg/mL. The regression equation was y=0.1493x+0.0583, R²=0.9996. The best technology of water extraction was soaking for 45 min, adding 10 times water, decocting 3 times and 45 min each. Considering the practical production and cost factors, the water extracting technology of Chinese herbal compound was soaking 30 min, adding 10 times water, decocting 3 times and 30 min each time which all closed to the maximum result in the orthogonal design.

Key words: berberine hydrochloride; yellow oral liquid; UV method