猪细小病毒疫苗株NS1基因克隆及生物信息学分析

Abstract

Abstract: In order to study the NS1 protein founction of porcine parvovirus(PPV) further, a pair of primers was designed according to PPV strain NADL-2 NS1 gene. The whole NS1 gene was obtained by PCR, and cloned into pTA2 to sequence. Sequence analysis result indicated that the recombinant plasmid(pTA2-NS1) contained one open reading frame with 1989 bp encoding 662 aa. Compared with PPV strains NADL-2 and Kresse, nucleotide homologies were 99.85% and 99.65%, putative amino acid homologies were 99.70% and 99.70%, respectively. Bioinformatics software(e.g. DNAStar) and net servers were applied to predict and analyze the structures and biochemical properties of NS1 protein. The results showed that the molecular weight of the protein NS1 was predicted as 75.7 ku, the isoelectric point PI was 6.82. It was a soluble protein, mainly located in the nucleus, containing neither signal peptide nor transmembrane region, with T199, Y309, T338, T512, S631, T635 being the potential phosphorylation sites. The construction of pTA2-NS1 and bioinformatics analysis laid the foundation for further functional research.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">porcine parvovirus; NS1 gene; bioinformatics analysis

LI Xing-yu, LI Jin-hai, LIN Yi, CAO Ye, LUO Dan-dan, LIAO Dang-jin, WEI Yong, LI Jiang-ling, YU Ji-feng, ZHANG Xian-hui. Cloning and Bioinformatics Analysis of NS1 Gene of Porcine Parvovirus Vaccine Strain[J]. .

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Cloning and Bioinformatics Analysis of NS1 Gene of Porcine Parvovirus Vaccine Strain

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