Abstract: In order to improve the growth performance of Guizhou Black goat, the SNP site in the promoter region of CISH gene in goat was screened and the effect of SNPs on function elements of promoter was analyzed. Guizhou Black goat were selected to construct DNA pools, SNP site was screened by direct sequencing subsequently. Bioinformatics tools were used to predict the core region of the promoter, transfer factor and CpG island. 1 single nucleotide polymorphism(SNP) G-95C was found in the 5′-flanking region of CISH gene. Furthermore, the core region of the promoter and CpG island were obtained. It demonstrated that various novel transcription factors binding sites emerged while a large amount of transcription factors binding sites existed before disappeared based on the SNP found in this study, while it didn’t affect the range of CpG island dramaticlly by using various softwares. There was one SNP existed in the 5′-flanking region of CISH gene that had important effects on promoter elements. This study could lay the experimental foundation for the identification of function of CISH promoter.

Key words:

Guizhou Black goat; CISH; SNP; promoter