京海黄鸡甲状腺激素应答蛋白Spot14α基因外显子1多态性与屠体和腹脂性状的相关性分析

Abstract

Abstract: The objective of this study was to find out the SNPs of THRSPα gene and analysed their effects on the carcass and abdominal fat traits in Jinghai Yellow chicken. This experiment aimed at the exon region of THRSPα gene, using PCR-SSCP combined with sequencing technology to detect the SNPs in 379 Jinghai Yellow chickens at 140 days old. We foud that there was a 9 bp del/ins mutation in the exon 1 area of the gene. For the THRSPα gene, three genotypes(AA, AB and BB) were detected in Jinghai Yellow chicken. The frequencies of A and B alleles were 0.4485 and 0.5515, respectively. The results of the correlation analysis showed that the polymorphsim site had no significant influence(P>0.05) on the 140 day weight, carcass(including carcass weight, head weight, feet weight, wing weight, chest muscle weight, leg muscle weight, eviscerated weight, half eviscerated weight) and abdominal fat traits. Therefore,we concluded that the polymorphsim site could’t be used as a potential marker of carcass and abdominal fat traits in Jinghai Yellow chicken molecular marker-assisted breeding.

Key words:

THRSPα gene；exon；polymorphism；Jinghai Yellow chicken；carcass and abdominal fat traits

XUE Qian,WANG Jin-yu,ZHANG Gen-xi, WANG Ya-nan,TANG Ying，WEI Yue,SHI Hui-qiang. Relationship between Polymorphisms of Exon 1 of THRSPα Gene and the Carcass and Abdominal Fat Traits in Jinghai Yellow Chicken[J]. .

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Relationship between Polymorphisms of Exon 1 of THRSPα Gene and the Carcass and Abdominal Fat Traits in Jinghai Yellow Chicken

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