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Study on Two Patterns Comparison of Gold Immunochromatography Test Strip for Rapid Detection of Lincomycin

ZHOU Jie1，XU Fei1，WU Chao1，LIU Wen-li1，DING Shuang-yang1，2   

  1. 1.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China；2.National Reference Laboratories for Veternary Drug Residue, Beijing 100193, China

  • Received:2014-01-08 Online:2014-07-20 Published:2014-08-21

Abstract: The objective of this study was to develop gold immunochromatography test strips to detect lincomycin in milk. Based on two patterns of direct competitive inhibition and indirect competitive inhibition, we optimized concentration of immunogold labeling BSA-LIN （IgG）, pH, concentration of coating IgG （BSA-LIN）, pretreatment of milk and so on.The results showed that we got two strips that fulfil practical requirement. The LOD of direct and indirect were 30 and 10 ppb. Comparing two methods, indirect method was better than direct in sensitivity, stability and testing time.

Key words:

lincomycin; gold immunochromatography; direct competition; indirect competition; milk