Abstract: This study was enforced to acquire and analyze sequence of goat CTSL gene, and to uncover the expression of CTSL mRNA in different tissues of goat. On the basis of other species mRNA, the cDNA of CTSL gene in goat was amplified successfully by using RT-PCR. The structure and function of goat CTSL gene were analyzed by bioinformatics ways. The mRNA abundance of goat CTSL gene was detected in 11 tissues. The results revealed that goat CTSL gene cDNA code area was 1005 bp in length and encoded 334 amino acids. The amino acids homology of goat CTSL were 99.10%, 96.71%, 90.12%, 76.95%, 76.12%, 75.82%, 66.17% and 61.42% comparing to that of the ovis aries, cattle, pig, human, rattus, mouse, medaka and zebra fish, respectively. The CTSL amino acid sequence of goat included a Inhibitor_129 domain and a Pept_C1 domain. The mRNA expression level of goat CTSL gene was tested in all 11 tissues, but it was significantly higher in lung and in kidney than in heart, liver, spleen, pectoral muscle, abdominal muscle, crureus, diaphragm, ocular muscle and soleus（P<0.05）.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">goatfont-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">；CTSL gene；cloning；sequence analysis；tissue expression