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Molecular Cloning of cDNA Sequences Encoding TGFα Gene in the SD Rats

ZHANG Ming-hao1, ZHANG Jin-tao2, LI Ya-ping3   

  1.  (1. Medical Science Function Laboratory, Henan University of Traditional Chinese Medcine, Zhengzhou 450046, China；2. Laborator Animal Center, Zhengzhou University, Zhengzhou 450052, China；3. Drug Affairs Department, Zhengzhou Hospital of Traditional Chinese Medcine, Zhengzhou 450007, China)

  • Received:2013-10-28 Online:2014-06-20 Published:2014-07-24

Abstract: This study was designed to clone and analyze the cDNA encoding TGFα from SD rats. The PCR method was developed to clone the TGFα cDNA. A full-length cDNA and CDS sequences of SD rat were 970 and 483 bp, which had been accepted by GenBank (accession number: KF366251). TGFα protein encoded by this gene was composed of 160 amino acid residues. The identities of coding sequences of TGFα gene were 98.6%, 95.0%, 85.5%, 85.3%, 85.5%, 86.3%, 86.3%, 73.2%, 73.2% and 56.8% by homologous comparison among SD rat and other species, and in amino acid sequences were 98.8%, 96.9%, 90.6%, 90.6%, 91.2%, 91.9%, 91.9%, 72.6%, 72.6% and 38.8%, respectively. The results suggested a certain degree of conservation of the TGFα gene among different species, but there were some specificity in function among different species. The results derived from information searching by BLAST program revealed there were 7 SNPs sites in the sequences of TGFα gene coding sequences between SD rats and BN rats in GenBank. The results provided new data for SNPs in TGFα gene.

Key words:

rat；TGFmso-bidi-font-family: 宋体">α gene；molecular cloning；sequence analysis