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Clone and Expression Analysis of AdipoQ Gene in Tibetan Goat

LI Xue-mei, XUE Ke, ZHONG Tao, LI Li, WANG Lin-jie, ZHANG Hong-ping   

  1.  (Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China)

  • Received:2013-11-27 Online:2014-06-20 Published:2014-07-24

Abstract: In order to study the expression pattern and functions of AdipoQ, we used the bioinformatics and comparative genomics methodologies to clone the Tibetan goat AdipoQ gene. Semi-quantative RT-PCR and fluorescence quantification PCR were performed to analyse the AdipoQ mRNA expression pattern and relative expressions in three different adipose tissues of Tibetan goat. The results showed that the AdipoQ gene in Tibetan goat had high identity with the orthologous gene in other mammals. We found that AdipoQ was highly expressed in adipose tissue, and weakly expressed in skeletal muscle and rumen, and no expression was detected in other tissues. The expression of AdipoQ in perirenal fat tissue was higher than those in subcutaneous fat tissue and mesenteric fat tissue, but the expression difference was not significant (P>0.05). In this study, we obtained the sequence and tissue expressions of AdipoQ gene which laid a foundation for further studying its functions in Tibetan goat.

Key words:

AdipoQ; Tibetan goat; fat deposition