Abstract: One strain of Staphylococcus aureus was isolated and identified by 16S rRNA sequence analysis and biochemical analysis in a large-scale sheep farms, it was named as JLY-1. The drug resistance analysis of the strain was conducted. The result showed that JLY-1 was highly resistant to erythromycin, ampicillin, chemitrim (MIC≥512 μg/mL)；resistant to doxycycline, amikacin (MIC≥64 μg/mL) and ciprofloxacin, enrofloxacin (MIC≥4 μg/mL)；and sensitive to florfenicol and cefatriaxone (MIC≤0.25 μg/mL).

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">sheepfont-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">；Staphylococcus aureus；isolation and identification；drug resistance