弧菌颉颃菌的筛选及其在对虾养殖中的初步应用

Isolation and Effect of Antagonistic Bacteria against Vibrio in Shrimp Aquaculture

WANG Jiao¹，QU Peng¹，SUN Fei¹，TIAN Xiang-li²，LIANG Jing-jing¹，CAI Yu-yong¹

（1.Qingdao Genyuan Biotech Group，Qingdao 266736，China；2.Key Laboratory of Mariculture，Ministry of Education，Ocean University of China，Qingdao 266003，China）

Received:2013-12-27 Online:2014-06-20 Published:2014-07-24

Abstract

Abstract: A total of 22 strains were isolated from aquatic environment.The potential probiotic strains were selected by dot inoculating method to screen the bacteria with antagonism property. The isolate A4 was identified as Bacillus subtilis based on conventional biochemical tests and 16S rRNA sequence homology， which exhibited significantly inhibitory activity against Vibrio parahaemolyticus，Vibrio alginolyticus and Vibrio brilliant.The probiotic effect of Bacillus subtilis showed that it could reduce the levels of the pathogenic bacteria and affect shrimp health after two days of culture （P＜0.05）.The Bacillus subtilis had potential applications for controlling pathogenic bacteria，which could be used as biocontrol agents in the aquaculture industry.

WANG Jiao，QU Peng，SUN Fei，TIAN Xiang-li，LIANG Jing-jing，CAI Yu-yong. Isolation and Effect of Antagonistic Bacteria against Vibrio in Shrimp Aquaculture[J]. .

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Isolation and Effect of Antagonistic Bacteria against Vibrio in Shrimp Aquaculture

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