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Expression Changes of Dab2 in Kidney Tissue of the Aristolochic Acid Nephropathy Mice

WU Jin-dong, CHANG Di, YANG Yao-hui, TIAN Ya-hui, ZHAO Hai-jiao, WU Guo-juan   

  1.  (Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China)

  • Revised:2014-01-24 Online:2014-06-20 Published:2014-07-24

Abstract: The study was aimed to observe the expression changes of Dab2 in kidney tissue of the aristolochic acid nephropathy (ANN) mice. In the foundation of ANN mice model, utilizing the immunohistochemistry, Real-time fluorescence quantitative PCR (qRT-PCR) and Western blotting technology to detect the expression changes of Dab2 in kidney tissues. With the extension of treatment time, the kidney structure was more seriously damaged, the expression change of Dab2 decreased on the 6th day (P>0.05), suddenly increased on the 12th day and extremely significantly decreased (P<0.01), but the overall trend of Dab2 expression change decreased, and the change of mRNA was consistent with this;at the same time, the location of Dab2 proteins changed, transferred from the brush border to the cytoplasm, and transferred into the nucleus of tubular epithelial cells. This study showed that Dab2 involved in the progress of ANN, this study layed the foundation for the function research of Dab2 in the progress of AAN.

Key words:

Dab2; aristolochic acid nephropathy; kidney; expression