Abstract: The research was aimed to study the impacts of different feeding nutrients, start time of feeding and cell inoculation density on the growth of BHK-21 suspension cells.The result showed that amino acid concentrated liquid had a apparent influence on promoting the growth of BHK-21 suspension cells. But serum components played a more important role in the promotions of cell growth, maintenance and cell activity. When initial inoculation density was 3×10⁵ to 6×10⁵ cells/mL, adding a certain amount of concentrated liquid at 48 h, BHK-21 suspension cells grew well. In addition, BHK-21 suspension cells did not obviously occur apoptosis in the optimized conditions.

Key words: BHK-21 cells; suspension culture; fed batch culture; cell apoptosis