贝氏柯克斯体外膜蛋白H（OmpH）原核表达载体的构建与鉴定

Construction and Identification of Outer Membrance Protein H （OmpH） of Coxiella burnetii

QIAO Hai-yan¹^，2，JIA Guang-le¹，LIN Xiang-mei¹, ZHANG Ying²，LIAO Juan-hong¹

（1.Chinese Academy of Inspection and Quarantine, Beijing 100121，China；2.Shanxi Agricultural University，Taigu 030801，China）

Received:2013-10-12 Online:2014-05-20 Published:2014-06-25

Abstract

Abstract: This study was amied to construct and identify the prokaryotic expression plasmids of outer membrance protein H (OmpH) of Coxiella burnetii and analysis its immunogenicity. Amplified OmpH gene from the Nine Miles by PCR, the results showed that the OmpH included 798 bp. Cloned OmpH gene into prokaryotic expression vector pQE-30 after identified by PCR,enzyme digestion and sequence. Constructed the recombinant plasmid pQE-30/OmpH,and then the plasmid was transformed into BL21(DE3) competent cells and induced by IPTG. The results showed that pQE-30/OmpH recombinant proteins were 25 ku by SDS-PAGE， and Western blotting assay indicated that the recombinant protein could express OmpH protein with significant antigencity.

Key words:

Coxiella burnetii; outer membrance protein H （OmpH）; prokaryotic expression; immunogenicity

QIAO Hai-yan，JIA Guang-le，LIN Xiang-mei, ZHANG Ying，LIAO Juan-hong. Construction and Identification of Outer Membrance Protein H （OmpH） of Coxiella burnetii[J]. .

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Construction and Identification of Outer Membrance Protein H （OmpH） of Coxiella burnetii

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