不同品种绵羊皮肤毛囊中角蛋白关联蛋白8-1基因差异表达研究

Study on Differential Expression of Keratin Associated Protein 8-1 Gene of Different Varieties of Ovis aries in Skin and Hair Follicle

ZHANG Gui-shan¹，XUE Jing-long¹，SUN Li-min¹，ZHAO Jia¹，XU Jing¹^，2，JIANG Huai-zhi¹

（1.College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，China；2.Changchun University of Science and Technology，Changchun 130600，China)

Received:2013-10-31 Online:2014-05-20 Published:2014-06-25

Abstract

Abstract: Relative expression of keratin associated protein 8-1 （KAP8-1）gene was determined in different vrieties of Ovis aries skin and hair follicle so as to identify the regulation of KAP8-1 gene on skin and hair follicle development and wool quality difference. Using Real-time PCR SYBR GreenⅠ, Ct of expression of KAP8-1 gene was determined and relative expression of KAP8-1 gene was analyzed by 2^-△△Ct. The results showed that there was KAP8-1 gene expression in there different crossbred varieties of Ovis aries (South African mutton Merino sheep（♂）× Northeast Fine wool sheep（♀），South African mutton Merino sheep（♂）× Small-tailed Han sheep（♀），Dorper sheep（♂）× Small-tailed Han sheep（♀）) skin and hair follicle. The ratio of relative expression was 7.56∶2.19∶1. In a word, KAP8-1 gene played a role in regulating skin and hair follicle development and wool quality difference,with difference among different varieties.

Key words:

Ovis ariesmso-bidi-font-family: 宋体">；skin and hair follicle；keratin associated protein 8mso-bidi-font-family: 宋体" lang="EN-US">-1(KAP8mso-bidi-font-family: 宋体" lang="EN-US">-1)；relative expression

ZHANG Gui-shan，XUE Jing-long，SUN Li-min，ZHAO Jia，XU Jing，JIANG Huai-zhi. Study on Differential Expression of Keratin Associated Protein 8-1 Gene of Different Varieties of Ovis aries in Skin and Hair Follicle[J]. .

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Study on Differential Expression of Keratin Associated Protein 8-1 Gene of Different Varieties of Ovis aries in Skin and Hair Follicle

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