Abstract: The gB and gD antigen epitope genes of pseudorabies virus (PRV) Fujian strain(LY strain) were amplified by PCR, the paraments of gB and gD were cloned into pMD18-T easy and sequenced,getting about 578 and 653 bp DNA fragments, then the gB and gD gene of PRV LY strain were cloned and sequenced. Compared with gB and gD antigen epitope genes in GenBank, the sequence analysis determination show that the gB and gD gene of LY strain shares 74.9% to 98.8% and 97.2% to 98.1% nucleotide homology, 62.9% to 96.4% and 94.5% to 96.8% amino acid sequence homology with other strains. The evolutionary analysis indicated that LY strain had the same evolutionary branch with domestic epidemic strains, and had a close relationship with the Ea strain (Hubei), LA strain (Shandong) isolated from domestic strains other than from abroad.

Key words: font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">PRVfont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">；gB gene；gD gene；sequences analysis