Abstract: A poultry farm suffered an unknown disease suspected as fowl cholera in Hubei province. A bacterium strain was isolated from liver and lung samples. The isolates was identified as Pasteurella multocida by morphological and cultural characteristics, biochemical properties, and sequence alignment based on 16S rRNA, and named as Pasteurella multocida -HB strain. 30 day old SPF chickens were used to test the pathogenicity and the result showed the strain was deadly to chickens at very low dose, 10 CFU per chicken.

Key words: font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Pasteurella multocidafont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">；isolation；identification；16S rRNA