Abstract: In order to understand the infection status of 3 kinds of bovine viral diarrhea pathogens，bovine viral diarrhea virus (BVDV)，bovine rotavirus (BRV) and bovine coronavirus (BCV) in Qinghai，RT-PCR method was adopted for nucleic acid detection and analysis of BVDV，BRV，BCV on clinical materials 32 with diarrhea and 152 healthy cattle feces samples which collected from 2012 to 2013 in part area of Qinghai province.The results showed that 32 diarrhea disease of cattle feed samples BVDV，BRV，BCV positive rate were 65.63% (21/32)，18.75% (6/32)，34.38% (11/32)，respectively, mixed with 2 or 3 species infection.152 healthy cattle feces samples BVDV，BRV，BCV positive rate were 3.95% (6/152)，1.97% (3/152)，0(0/152)，respectively.These results indicated that BVDV，BRV，BCV widespread infection in cattle in some areas of Qinghai，and mixed infection was serious，which need to further strengthen the comprehensive prevention and control of bovine viral diarrhea pathogens in Qinghai province.

Key words:

bovine viral diarrhea virus; bovine rotavirus; bovine coronavirus; infection; investigation