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Oocyte Recovery, in vitro Maturation and Embryo Culture Systems Influence the Efficiency of Ovine in vitro Fertilization

MIN Jiang-tao1,2, DU Wei-hua2, ZHAO Xue-ming2, HAO Hai-sheng2,HANG Su-qin1, ZHU Hua-bin2   

  1. 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

  • Received:2013-12-03 Online:2014-05-20 Published:2014-06-25

Abstract: Taking advantage of the ovaries from slaughterhouse, the effects of different kinds of oocyte recovery methods (follicle flushing, slicing, syringe aspiration and pump aspiration), different kinds of gonadotropins (FSH/LH from BIONICHE and Ningbo hormone factory) and serums (estrous sheep serum or fetal bovine serum) during in vitro maturation and different embryo culture systems (mCR and mSOF) on the efficiency of ovine in vitro fertilization were investigated. The results showed that the percentage of grade A and B oocytes recovered using the flushing method was 77.1%, which was significantly higher than using the other methods (P<0.05). In vitro maturation rate of oocytes matured in the medium supplemented with estrous sheep serum (ESS) and FSH/LH from BIONICHE was significantly higher than the other treatments (P<0.05). In mSOF and mCR cultural system, there was no significant difference in the cleavage rate between two groups (P>0.05). However, mSOF provided significantly higher rates of blastocyst and hatched blastocyst than mCR (P<0.05). These results demonstrated that the flushing method introduced in present study was more appropriate for ovine oocyte recovery than other recovery methods;The maturation rate of ovine oocytes could be increased significantly with the supplementation of ESS and BIONICHE FSH/LH into in vitro maturation media;Compared with mCR, mSOF was superior for culturing ovine IVF embryos.

Key words:

ovine IVF; oocyte recovery; oocyte IVM