Abstract: This study was to make fermentation bed by the cotton stalk instead of sawdust and rice husk，the combination of substrate included cotton stalk 65%，sawdust 7%，rice husk 28%,using 4 kinds of media （PDA medium （PDA），sabouraud medium （S），niushi medium （N），carboxymethyl cellulose sodium medium（SK）） and extracted five kinds of strains from different materials （cotton stalk rot，decay wood，decaying vegetation，manure，pig fermentation bed），the subject worked on of three experiments from the combination of substrate，the ratio of strain and the carrying capacity of fermentation bed using microbiology technology. The results showed that the ratio of PDAs∶Sf∶Nf∶Nb∶SKm was 4∶2∶2∶1∶1，adding strains was 10%，each pig’s share of area was 1.2 m²，it was the best to use of the fermentation bed and paid a solid base for the production of fermentation bed with cotton stalk.

Key words: cotton stalk; fermentation bed; combination of substrate; ratio of strain; carrying capacity