牛乳中α-酪蛋白完全抗原的合成及多克隆抗体的制备

Abstract

Abstract: In order to gain polyclonal antibody against α-casein, the commercial α-casein were linked with carrier protein BSA and OVA, respectively， as immunogen (α-casein-BSA) and coating antigen (α-casein-OVA). The reaction products were identified by nondenaturing PAGE and SDS-PAGE. New Zealand White rabbits were immunized with the immunogen (α-casein- BSA) to produce polyclonal antibody. The results showed that the titer of the antibody was 1∶256000 and had high cross reactivity with κ-casein. It could be used to detect the total amount of α-casein and κ-casein in milk.

Key words: α-casein; complete antigen; polyclonal antibody

CHU Xiao-hong, ZHOU Yu, LI Yan-song, MENG Xing-yu, ZHANG Ying, LU Shi-ying, REN Hong-lin, HU Pan, LIU Zeng-shan, WANG Nan-nan, LI Dan. Synthesis of Complete Antigen and Preparation of Polyclonal Antibody against α-casein in Bovine Milk[J]. .

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Synthesis of Complete Antigen and Preparation of Polyclonal Antibody against α-casein in Bovine Milk

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