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Expression of the Recombinant Plasmid pMEF2A in Fetal Lamb Myoblasts

CHEN Fu-ying1, CHU Qiu-xia1, JIA Rong-ling2, NIU Hui3, WU Jiao4, WANG Er-yao1,FENG Ya-jie1, XU Zhao-xue1   

  1.  (1. Institute of Animal Husbandry and Veterinary Medicine, Henan Academy of Agricultural Sciences, Zhengzhou 450002，China；2. Nanyang Vocational School of Agriculture, Nanyang 473000，China；3. Research Center of Cattle Engineering Technology in Henan, Zhengzhou 450003，China；4. Institute of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450003，China)
  • Received:2013-08-06 Online:2014-02-20 Published:2014-03-27

Abstract: The CDS of cattle myocyte enhancer factor-2 A (MEF2A) was amplified by RT-PCR and cloned into eukaryotic expression vector pEGFP-C1 and transfected into fetal lamb myoblasts, meanwhile transfection empty vector as control. The fluorescence intensity of transcription was detected by the reporter gene after transfection. The results indicated that the fluorescence intensity of cells with recombinant plasmid than that of cells with blank vector after 48 h transfection. By selection with G418, the stably transfected cell lines with blank vector were detected while cells with the screening condition of cells with recombinant plasmid need further optimize.

Key words: MEF2A gene; myoblasts; eukaryotic expression