牛病毒性腹泻病毒实时荧光定量RT-PCR方法的建立及应用

Abstract

Abstract: Persistent infection and immunologic tolerance are important characteristics of bovine viral diarrhea disease, which have caused great difficulties on the diagnosis, quarantine and prevention of the bovine viral diarrhea virus (BVDV), so a fast and efficient detection method of BVDV is quite necessary. A pair of primers were designed based on the gene sequences of BVDV 5′UTR published in GenBank, then established the Real-time fluorescent quantitative RT-PCR detection method with SYBR Green Ⅰ to detect BVDV. The results showed that the detection method had high specificity, strong sensitivity and good repeatability. Thus, the detection method of the Real-time fluorescent quantitative RT-PCR would provide an effective means for the rapid detection of BVDV and persistent infected animals. It could supplement and perfect the quarantine and diagnostic methods of BVDV.

Key words:

bovine viral diarrhea virus (BVDV); Real-time fluorescent quantitative RT-PCR; 5′UTR; detection method

WANG Rong, LI Wen-wen, WANG Yan, LIU Ya-gang, YU Qiong, REN Yong-gang. Establishment and Application of Real-time Fluorescent Quantitative RT-PCR Method for Detection of Bovine Viral Diarrhea Virus[J]. .

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Establishment and Application of Real-time Fluorescent Quantitative RT-PCR Method for Detection of Bovine Viral Diarrhea Virus

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