(1.Guangdong Entry-exit Inspection and Quarantine Bureau,Guangzhou 510623,China;2.School of Life Sciences,Sun Yat-Sen University,Guangzhou 510275,China;3.Research Institute of Veterinary Medicine,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;4.National Research Institute for Family Planning,Beijing 100081,China)
1 王军一，金扩世，徐敬龙.山东种公猪精液携带主要病原的检测[J].动物医学进展，2012，33(8):108～111.2 张敬友.进境猪精液风险分析[D].南京:南京农业大学，2005.3 殷震, 刘景华.动物病毒学[M].第2版.北京:科学出版社，1997.4 袁亚男，刘文忠.实时荧光定量PCR技术的类型、特点与应用[J].中国畜牧兽医，2005，35(3):27～30.5 龚黎明，葛琼，卢义愚，等.4种核酸提取方法在流行性感冒病毒核酸检测中的比较[J].中国卫生检验杂志，2007，17(1):53～57.6 韩一超，吴忻，姚敬明，等.4种猪繁殖障碍性疫病流行病学调研与分析[J].中国畜牧兽医，2011，38(11):195～200.7 楼平儿（译）. 通过人工授精传播的猪疾病[J].猪业科学，2012，29(7):100～102.8 魏占勇，梁静怡，李厚伟，等.精液中猪细小病毒DNA三种提取方法效果比较[J]. 浙江农业学报，2009，21(5):455～458.9 Bauer M, Patzelt D. A method for simultaneous RNA and DNA isolation from dried blood and semen stains[J].Forensic Science International, 2003, 136(1～3):76～78.10 Christopher-Hennings J, Dammen M, Nelson E, et al. Comparison of RNA extraction methods for the detection of porcine reproductive and respiratory syndrome virus from boar semen[J]. Journal of Virological Methods, 2006, 136(1～2):248～253.11 Hebia-Fellah I, Leaute A, Fieni F, et al. Valuation of the presence of equine viral herpesvirus 1 (EHV-1) and equine viral herpesvirus 4 (EHV-4) DNA in stallion semen using polymerase chain reaction(PCR)[J].Theriogenology, 2009, 71(9):1381～1389.12 Holzapfel B, Wickert L. Die quantitative Real-Time-PCR (qRT-PCR)[J].Methoden und Anwendungsgebiete, 2007(37):120～126.13 Medveczky I, Szabo I.Isolation of Aujeszky’s disease virus from boar semen[J].Acta Vet Acad Sci Hung, 1981, 29(1):29～35.14 Van Rijn P A, Wellenberg G J, Hakze Van Der Honing R, et al. Detection of economically important viruses in boar semen by quantitative RealTime PCR technology[J].Journal of Virological Methods, 2004, 120(2):151～160.
