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Differential Expression of microRNAs in Cerebellum of SPRN0/0 Mice and C57BL/6 Wild Mice

BAI Huan-li1，2, ZHANG Heng1，4, JU Chuan-jing1，3，MENG Ke-yin1，LI Zhong-yi1，ZHANG Xiao-li1，5, ZHANG Teng-long1，4, BO Le1，4, WANG Xiong4, CHEN Zhi-bao4,WAN Jia-yu1,SHEN Hai-qing2   

  1. （1. Military Veterinary Institute, Academy of Military Medical Sciences of PLA, Changchun 130122, China；2.College of Animal Medicine, Jilin University,Changchun 130062,China；3.The Forth Hospital of Jilin University, Changchun 130011, China；4. College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163000, China；5. College of Life Science and Technology, Changchun Polytechnic University, Changchun 130022, China）
  • Received:2013-09-05 Online:2014-02-20 Published:2014-03-27

Abstract: The purpose of this study was to identify different expression of microRNAs between cerebellum of SPRN0/0 mice and C57BL/6 wild mice, and investigate relative expression results of microRNA in cerebrum of SPRN0/0 mice by Real-time PCR technology. The results showed that compared with cerebellum of C57BL/6 wild mice, eight microRNAs were decreased significantly:miR-135a, miR-24-2*, miR-146a, miR-7a, miR-380, miR-448, miR-128 and miR-152. miR-24-2* fold change to more than 10, five microRNAs including miR-448，miR-146a，miR-128，miR-380 and miR-152 fold change to more than 2, miR-135a and miR-7a fold change to more than 1. Thus, we concluded that these changes of microRNAs expression might be involved in Shadoo protein function and the establishment of target genes of microRNAs might provide new ideas for researching Shadoo protein function.

Key words: Shadoo protein; microRNA; Real-time quantitative PCR; cerebellum