羊种布鲁氏菌Omp10基因的克隆及原核表达

Abstract

Abstract: According to the Brucella melitensis vaccine strain M5-90 outer membrane protein 10 (Omp10) genetic sequence in GenBank, a pair of primers was designed and the PCR technology was used to amplify Omp10 with its whole genome as the template. The Omp10 fragment which was 381 bp was obtained. Insert it into the plasmid pMD20-T, and it was transformed into E.coli DH5α. Recombinant prokaryotic expression plasmid, pET-28a-Omp10 was constructed, and transformed into E.coli BL21(DE3), and the fusion protein His-Omp10 was induced and expressed by IPTG, and identified with SDS-PAGE and Western blotting to analysis. The results showed that the recombinant protein was expressed successfully, which laid a solid foundation to the study of the animal immunization test profoundly.

Key words: Brucella melitensis; Omp10 gene; prokaryotic expression; cloning

CLC Number:

R378.5

XU Kai-lian, ZHU Hua-pei, ZHAO Tian-jing, JIA Xiao-xiao, GUO Shi-yu, PANG Feng, JIAO Han-wei, CHENG Ying, DU Li, SHI Qiao-yun, RONG Hui, ZHANG Jia-ning, WANG Feng-yang. Cloning and Prokaryotic Expression of Omp10 Gene of Brucella melitensis[J]. , 2014, 41(12): 122-125.

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Cloning and Prokaryotic Expression of Omp10 Gene of Brucella melitensis

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