6株羊源乳酸杆菌电转化条件的优化

Abstract

Abstract: This study was aimed to establish the optimal electrotransformation conditions of six Lactobacilli strains (L.plantarum, L.casei, L.reuteri, L.brevis, L.coryniformis and L.curvatus) which isolated from Shanbei White cashmere goats' rumen, plasmid pLEM415-egfp was electrotransformed into competent cell of six Lactobacilli on different growth phase (D_{600 nm}=0.4, 0.6, 0.8, 1.0 and 1.2), and the electrotransformation efficiency was investigated under resistance 200 Ω, capacitance 25 uF and different electric field strength (1.5, 2.0 and 2.5 kV). Results showed that the optimal D_{600 nm} and electric field strength to obtain the maximum transformation efficiency for L.plantarum, L.casei, L.reuteri, L.brevis, L.coryniformis and L.curvatus were 0.8, 1.2, 1.0, 0.6, 0.8, 0.4 and 1.5, 1.5, 1.5, 1.5, 1.5, 2.0 kV, respectively. The establishment of optimal electrotransformation conditions for six Lactobacilli strains provided the foundation for further construction and application of transgenic Lactobacillus.

Key words: Lactobacillus; growth phase; electric field strength; electrotransformation; transformation efficiency

CLC Number:

S826

FANG Ming-jian, WANG Lei, CHEN Yu-lin. Optimication of Electrotransformation Conditions of Six Lactobacilli Strains[J]. , 2013, 40(9): 80-85.

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Optimication of Electrotransformation Conditions of Six Lactobacilli Strains

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