›› 2013, Vol. 40 ›› Issue (9): 105-108.

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Research Progress on Yeast Two-hybrid Technology and Application

ZHENG Li-shuang1, LI Xiang-nan2, SUN Cheng-tao2, LIU Hong-yu2, LIU Xun2, HE Ming2, LV Wen-fa2   

  1. 1. College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;
    2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
  • Received:2013-01-28 Online:2013-09-20 Published:2013-09-18

Abstract: Yeast two-hybrid technology is an effective molecular biology method which is used to study the interaction of proteins. It is relatively simple, fast, efficient, sensitive, extensive and can directly detect the interaction of intracellular proteins. This paper mainly introduces the basic principle, applications, advantages and disadvantages of yeast two-hybrid technology, now the research on yeast two-hybrid system technology and application will be reviewed.

Key words: yeast two-hybrid system; eukaryotic cell; protein

CLC Number: