杆状病毒表达系统在兽用亚单位疫苗领域应用的研究进展

Abstract

Abstract: Baculovirus expression system, based on baculovirus as the protein expression vector and insect cells or larvas as the host, is an eukaryotic expression system. In comparison with prokaryotic cells, the insect cells permit post-translational modfications of foreign proteins, such as folding, glycosylation, acylation, phosphorylation, disulfide bond formation, proteolytic cleavage and so on. Moreover, the baculoviruses cannot infect livestock. These advantages make baculovirus expression system be a safe and efficient platform for the production of veterinary subunit vaccines. Here, we critically review the baculovirus expression system as a platform to produce veterinary subunit vaccines.

Key words: baculovirus expression system; baculovirus; insect cell; veterinary subunit vaccine; virus-like particle; processing

CLC Number:

S852.65

LIU Chun-ju, REN Wei-jie, WANG Zhi-liang. Research Progress on Application of Baculovirus Expression System in Veterinary Subunit Vaccine Field[J]. , 2013, 40(9): 101-105.

References

1 Belyaev A S, Roy P. Development of baculovirus triple and quadruple expression vectors: Co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells[J]. Nucleic Acids Research, 1993, 21(5): 1219~1223.
2 Cox M M. Recombinant protein vaccines produced in insect cells[J]. Vaccine, 2012, 30(10): 1759~1766.
3 de Diego A C P, Athmaram T N, Stewart M, et al. Characterization of protection afforded by a bivalent virus-like particle vaccine against bluetongue virus serotypes 1 and 4 in sheep[J]. PLoS One, 2011, 6(10): e26666.
4 de Smit A J, Bouma A, De Kluijver E P, et al. Duration of the protection of an E2 subunit marker vaccine against classical swine fever after a single vaccination[J]. Veterinary Microbiology, 2001, 78(4): 307~317.
5 Fan H, Ju C, Tong T, et al. Immunogenicity of empty capsids of porcine circovius type 2 produced in insect cells[J]. Veterinary Research Communications, 2007, 31(4): 487~496.
6 Hawtin R E, Zarkowska T, Arnold K, et al. Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes[J]. Virology, 1997, 238(2): 243~253.
7 Hervas-Stubbs S, Rueda P, Lopez L, et al. Insect baculoviruses strongly potentiate adaptive immune responses by inducing typeⅠ IFN[J]. Journal of Immunology, 2007, 178(4): 2361~2369.
8 Jarvis D L. Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production[J]. Virology, 2003, 310(1): 1~7.
9 Latham T, Galarza J M. Formation of wild-type and chimeric influenza virus-like particles following simultaneous expression of only four structural proteins[J]. Journal of Virology, 2001, 75(13): 6154~6165.
10 Lee D H, Kwon J S, Lee H J, et al. Inactivated H9N2 avian influenza virus vaccine with gel-primed and mineral oil-boosted regimen could produce improved immune response in broiler breeders[J]. Poultry Science, 2011a, 90(5): 1020~1022.
11 Lee D H, Park J K, Lee Y N, et al. H9N2 avian influenza virus-like particle vaccine provides protective immunity and a strategy for the differentiation of infected from vaccinated animals[J]. Vaccine, 2011b, 29(23): 4003~4007.
12 Liu L J, Suzuki T, Tsunemitsu H, et al. Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus[J]. Archives of Virology, 2008, 153(12): 2291~2295.
13 Mena J A, Kamen A A. Insect cell technology is a versatile and robust vaccine manufacturing platform[J]. Expert Review of Vaccines, 2011, 10(7): 1063~1081.
14 Moormann R J, Bouma A, Kramps J A, et al. Development of a classical swine fever subunit marker vaccine and companion diagnostic test[J]. Veterinary Microbiology, 2000, 73(2~3): 209~219.
15 Pan Y S, Wei H J, Chang C C, et al. Construction and characterization of insect cell-derived influenza VLP: Cell binding, fusion, and EGFP incorporation[J]. Journal of Biomedicine & Biotechnology, 2010, 2010: 1~11.
16 Perez-Martin E, Gomez-Sebastian S, Argilaguet J M, et al. Immunity conferred by an experimental vaccine based on the recombinant PCV2 Cap protein expressed in Trichoplusia ni-larvae[J]. Vaccine, 2010, 28(11): 2340~2349.
17 Rueda P, Fominaya J, Langeveld J P, et al. Effect of different baculovirus inactivation procedures on the integrity and immunogenicity of porcine parvovirus-like particles[J]. Vaccine, 2000, 19(7~8): 726~734.
18 Smith G E, Summers M D, Fraser M J. Production of human beta interferon in insect cells infected with a baculovirus expression vector[J]. Molecular and Cellular Biology, 1983, 3(12): 2156~2165.
19 Sokolenko S, George S, Wagner A, et al. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks[J]. Biotechnology Advances, 2012, 30(3): 766~781.
20 Song J M, Hossain J, Yoo D G, et al. Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles[J]. Virology, 2010, 405(1): 165~175.
21 Song J M, Choi C W, Kwon S O, et al. Proteomic characterization of influenza H5N1 virus-like particles and their protective immunogenicity[J]. Journal of Proteome Research, 2011, 10(8): 3450~3459.
22 Stewart M, Bhatia Y, Athmaran T N, et al. Validation of a novel approach for the rapid production of immunogenic virus-like particles for bluetongue virus[J]. Vaccine, 2010, 28(17): 3047~3054.
23 Stewart M, Dubois E, Sailleau C, et al. Bluetongue virus serotype 8 virus-like particles protect sheep against virulent virus infection as a single or multi-serotype cocktail immunogen[J]. Vaccine, 2013, 31(3): 553~558.
24 Tomiya N, Narang S, Lee Y C, et al. Comparing N-glycan processing in mammalian cell lines to native and engineered lepidopteran insect cell lines[J]. Glycoconjugate Journal, 2004, 21(6): 343~360.
25 Tretyakova I, Pearce M B, Florese R, et al. Intranasal vaccination with H5, H7 and H9 hemagglutinins co-localized in a virus-like particle protects ferrets from multiple avian influenza viruses[J]. Virology, 2013, 442(1): 67~73.
26 Vicente T, Roldao A, Peixoto C, et al. Large-scale production and purification of VLP-based vaccines[J]. Journal of Invertebrate Pathology, 2011, 107(Suppl): S42~S48.
27 Wang C C, Chen J R, Tseng Y C, et al. Glycans on influenza hemagglutinin affect receptor binding and immune response[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(43): 18137~18142.
28 Wu P C, Lin W L, Wu C M, et al. Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development[J]. Applied Microbiology and Biotechnology, 2012, 95(6): 1501~1507.

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Research Progress on Application of Baculovirus Expression System in Veterinary Subunit Vaccine Field

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